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KMID : 0438219730100010371
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Korea University Medical Journal
1973 Volume.10 No. 1 p.371 ~ p.378
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Effects of Hypoxia and Hyperoxia on the Oxygen Consumption and Lactic Acid Production in the Brain and Kidney
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Abstract
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This experiment was performed in rabbits to investigate the effects of hypoxia and hyperoxia on the metabolism of brain and kidney tissues.
Experimental animals were divided into three groups; normal control group, hypoxic group induced by inhalation of 8% oxygen under one atmosphere and hyperoxic group induced by inhalation of 40% oxygen under one atmosphere for the time of 2 weeks exposed 5 hours per day in closed chamber. The inflow rates of each mixed gases were controlled by analysing the outflowed air with micro-Scholander apparatus of the O₂ and CO₂ contents.
At the end of exposure the rabbits were sacrificed with abrupt capitation and slices of cerebral cortex, cerebellum, medulla oblongata, thalamus and kidney cortex were prepared to determine the oxygen consumption with Warburg method and lactic acid concentration with the method of Barker and Summerson.
The results obtained were as follows:
1. In cerebral cortex slices, the oxygen consumption was not affected by hypoxia and hyperoxia, but the lactic acid concentration showed increasing tendency in both groups compared to control.
2. In cerebellar slices, oxygen consumption and lactic acid concentration showed no significant changes, or showed decreasing tendency.
3. Medulla oblongata showed no significant changes, however in the group of hyperoxia the lactic acid concentration was decreased.
4. In the region of thalamus, lactic acid was increased in hypoxia, however decreased in hyperoxia with similar oxygen consumption.
5. In the region of kidney cortex, the oxygen consumption revealed decrease in hypoxia, increase hyperoxia, and the lactic acid was decreased in hyperoxia, increased in hypoxia.
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KEYWORD
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